Minifor2Bio touch
Fermenter-Bioreactor

Just need an A4 sheet size of the bench space. Conventional bioreactor setups rely on bulky control towers, separate water bath recirculators, and a mess of external wiring. The LAMBDA Minifor2Bio touch integrates all control electronics, mass flow gas regulator, and heating elements into a single ultra-compact base unit with an A4 paper footprint (22" cm"×38" cm"×40" cm" ) and a total weight of just 7.5" kg" . This space-saving design allows up to four complete units to operate in the bench space typically required for a single conventional setup.

The Minifor2Bio touch eliminates heavy, cumbersome metal headplates and fragile flat O-rings in favor of Pyrex borosilicate glass vessels featuring threaded side necks set at a 30° angle. A central threaded neck accommodates the agitation mechanism, while 6 to 8 peripheral side necks host sensors, sampling lines, and condensers. Reusable silicone multi-seal stoppers deliver reliable gas-tight seals, simplify handling, and withstand repeated autoclaving to prevent contamination vectors.

Instead of internal resistance rods that cause thermal hot spots or heavy double-walled water jackets that require expensive glass vessel, heating is provided by a 150" W" IR heat source with a gilded parabolic reflector situated beneath the vessel. Half of the IR light is absorbed by the glass vessel wall and half directly by the liquid culture, mimicking natural sunlight. This delivers uniform, gentle heat distribution, fast response times, and precise temperature regulation (±〖0.2〗^∘ "C" ).

Inspired by nature, the Minifor2Bio touch uses an innovative up-and-down agitation with biomimicking fish-tail= discs. This simple yet highly effective mixing ensures easy sterility and thorough culture homogenization, all without complex magnetic coupling.

Working in harmony with the vertical movement, the agitator utilizes nature-inspired, biomimetic "fish-tail" stirring discs. Designed without sharp edges or high shear zones, these discs produce efficient horizontal and vertical fluid mixing with minimal micro-eddies. This gentle bulk movement protects shear-sensitive mammalian, insect, and plant cell lines while delivering strong oxygen mass transfer.

Conventional bioreactor headplates scale with vessel size, forcing laboratories to purchase dedicated headplate assemblies and varying probe lengths whenever scaling operations. The Minifor2Bio touch standardizes neck dimensions across all vessel capacities (0.3" L" , 0.4" L" , 1" L" , 3" L" , and 7" L" ). As a result, probes ("pH" , 〖"pO" 〗_2, temperature, antifoam) and accessory ports are interchangeable across all vessel sizes without purchasing duplicate sensors.

Maintaining long-term process sterility during extended continuous fermentations is simplified by removing all rotating mechanical penetrations. All sensors, feed lines, and gas outlets connect via elastic silicone stoppers and double-sealed multi-needle ports. The complete glass vessel assembly—including probes and tubing—can be autoclaved as a single unit without needing hot steam-in-place (SIP) utility lines.

A single Minifor2Bio touch base unit supports interchangeable Pyrex vessels ranging from micro-screening volumes (35" mL" ) up to full benchtop production capacities (6" L" ). Process screening, media development, and scale-up studies can be performed on the exact same console hardware.

The console features an ergonomic stepped "cascade" layout: the touchscreen display sits up front, the vessel rests on the main platform, magnetic reagent bottle holders mount behind, and feed pumps sit overhead. This arrangement guarantees 360° visual monitoring of the vessel, keeps liquid tubing connections short, and grants easy physical access to all side ports during inoculation and sampling.

Rigid glass or sintered metal spargers frequently clog when nutrient salts crystallize or media dries at the gas outlet. The system features an elastic silicone micro-sparger with dynamic micro-punctures. Under active gas flow, the elastic pores stretch open to deliver tiny bubbles for high oxygen transfer. When gas flow stops, the pores automatically snap shut to prevent medium backflow. Temporary gas pressure increases expand the elastic matrix to dislodge salt deposits directly into the medium.

Rather than using manual rotameters that fluctuate with pressure and temperature shifts, the unit incorporates an electronic thermal mass flow meter paired with a micro-proportional needle valve. It directly measures mass flow rate (0"-" 5" L/min" ) independent of backpressure variations. This enables continuous, automated feedback regulation of dissolved oxygen (〖"pO" 〗_2) by dynamically adjusting airflow or gas supply.

Minifor2Bio touch woth BALANCE 20 – Easy Weight Control: Maintains constant culture weight with an integrated scale module, enabling precise, reproducible continuous cultures.

Minifor2Bio touch integrates OD, turbidity, pH, pO₂, pCO₂, redox, conductivity, capacitance, and more—real‑time sensors for faster, reproducible results.

A corrosion-resistant design with minimal stainless steel exposure ensures reliable cultivation in aggressive and saline process environments.

Foam formation is sensed electrically via two adjustable vessel needles. When foam contacts the needles, the internal controller automatically triggers anti-foam agent delivery via a touch peristaltic pump or the ultra-compact LAMBDA DOZITO miniature syringe pump. The DOZITO delivers micro-doses to prevent chemical overdosing while preserving space around the vessel platform.

A cascade casing guarantees overall easy visibility, accessibility and handling. The display showing all parameters and control buttons is located in the front.

Reliable Connectivity for Efficient Data Management

Pumps specially developed for long term continuous processes like in fermentations and cell cultures. LAMBDA’s unique tubing compressing mechanics reduces pulsation and is very gentle to the tubing. No clamps, fixings or stoppers are required to hold the tubing in place. This leads to a long tubing life and the long term stability of flow rates even with low cost tubing.

LUMO programmable light controller with external LED light source for phototrophic cultures.

Easy calculation of OUR (Oxygen Uptake Rate), CPR (Carbon Dioxide Production Rate), and RQ (Respiratory Quotient)